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inactivated sto feeder cells  (ATCC)


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    ATCC inactivated sto feeder cells
    Inactivated Sto Feeder Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 453 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/inactivated+sto+cell+feeders/STO/pm30921588-33-15-19
    Average 95 stars, based on 453 article reviews
    inactivated sto feeder cells - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Comparison of different in vitro differentiation conditions for murine female germline stem cells
    Article Snippet: .. Briefly, FGSCs were cultured on mitomycin C‐treated (10 μg/mL, Sigma) mitotically inactivated STO cell feeders (derived from mouse SIM embryonic fibroblasts, strain SIM, 5 × 10 4 cells/cm 2 , ATCC). .. Culture medium for FGSCs was Minimum Essential Medium α (MEM‐α; Life Technologies) supplemented with 10% foetal bovine serum (FBS; Front), 1 m mol L −1 sodium pyruvate (Sigma), 1 m mol L −1 non‐essential amino acids (NEAA; Life Technologies), 2 m mol L −1 L‐glutamine (Sigma), 0.1 m mol L −1 β‐mercaptoethanol (Sigma), 10 ng/mL mouse leukaemia inhibitory factor (LIF; Santa Cruz Biotechnology), 10 ng/mL mouse epidermal growth factor (EGF; PeproTech), 40 ng/mL mouse glial cell line‐derived neurotrophic factor (GDNF; PeproTech), 10 ng/mL human basic fibroblast growth factor (bFGF; PeproTech)and 15 μg/mL penicillin.

    Derivative Assay:

    Article Title: Comparison of different in vitro differentiation conditions for murine female germline stem cells
    Article Snippet: .. Briefly, FGSCs were cultured on mitomycin C‐treated (10 μg/mL, Sigma) mitotically inactivated STO cell feeders (derived from mouse SIM embryonic fibroblasts, strain SIM, 5 × 10 4 cells/cm 2 , ATCC). .. Culture medium for FGSCs was Minimum Essential Medium α (MEM‐α; Life Technologies) supplemented with 10% foetal bovine serum (FBS; Front), 1 m mol L −1 sodium pyruvate (Sigma), 1 m mol L −1 non‐essential amino acids (NEAA; Life Technologies), 2 m mol L −1 L‐glutamine (Sigma), 0.1 m mol L −1 β‐mercaptoethanol (Sigma), 10 ng/mL mouse leukaemia inhibitory factor (LIF; Santa Cruz Biotechnology), 10 ng/mL mouse epidermal growth factor (EGF; PeproTech), 40 ng/mL mouse glial cell line‐derived neurotrophic factor (GDNF; PeproTech), 10 ng/mL human basic fibroblast growth factor (bFGF; PeproTech)and 15 μg/mL penicillin.

    Article Title: Production of offspring from a germline stem cell line derived from neonatal ovaries.
    Article Snippet: The idea that females of most mammalian species have lost the capacity for oocyte production at birth1–5 has been challenged recently by the finding that juvenile and adult mouse ovaries possess mitotically active germ cells6.. However, the existence of female germline stem cells (FGSCs) in postnatal mammalian ovaries still remains a controversial issue among reproductive biologists and stem cell researchers6–10.. We have now established a neonatal mouse FGSC line, with normal karyotype and high telomerase activity, by immunomagnetic isolation and culture for more than 15 months.



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